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["AP#*","备注","PI*","联系人*","手机*","email*","申请日期*","品系名称（全称）*","品系名称（简称）*","小鼠背景*","基因型","冻存方式*","雄鼠出生日期*","雄鼠编号*","雄鼠数量*","现在雄鼠所在笼位*","冻精复苏验证后代是否保留*","后代是否代管及转移房间*","后代剪尾是否有特殊要求*","雌鼠出生日期","雌鼠编号","雌鼠数量","现在雌鼠所在笼位号"]
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[null,null,"1.尽可能填写完整。（*为必填项，其他实验要求请填写在备注列）"]
[null,null,"2.保种方式的选择：我们采用精子冻存和胚胎冻存的方法进行保种。假如为杂合子，雄性数量≥3只，后代可以和B6交配，我们推荐安乐死1-2只雄鼠使用精子冻存的方式保种。"]
[null,null,"3.精子冻存对雄鼠的要求：Genotyping确定为阳性的雄鼠，2-3只，12-18周龄最佳。其他年龄也可以，主要看雄鼠健康状态。"]
[null,null,"4.雄鼠不再做试配：比如试配后冻存精子，受精率可以达成评价50-60%，不试配的话，是30%。实际看，只要冷冻后能复苏出后代，这个比例并不是最重要的。\n"]
[null,null,"5.胚胎冻存对小鼠的要求：雄鼠2-3只12-18W，雌鼠至少10只，最佳周龄为3-4W或9-12W，周龄大一些的也可以。正常情况下可冻存200枚胚胎左右。"]
[null,null,"6.冷冻后必须1-2周就开始复苏。冷冻的目的是减少或安乐死小鼠品系，我们在冷冻后1-2周液氮保存稳定期后进行复苏验证。默认胚胎期E14.5取胚胎做PCR验证，假如需要小鼠的新生小仔尾巴做PCR验证请一定说明。﻿"]
[null,null,"7.精子冻存实验会根据雄鼠周龄安排，复苏验证后通知具体数据，谢谢！"]
[null,null,"8.用于保种小鼠鉴定胶图请提供。"]